Atom Ant operated out of an anthill in the countryside, where he possessed such things as a mainframe computer and exercise equipment. His powers mostly consisted of the ability to fly, superspeed, and amazing strength.

He was often contacted by the police, who sent him out on assignment. Some of these missions parodied those of Batman. In one episode, the police force was shown to be underfunded and inept, as they relied on Atom Ant to do all their police work. The only two police officers were the chief and deputy chief. The department only possessed one rusted patrol car. Atom Ant fights various villians including recurring ones like Ferocious Flea and mad scientist Professor Von Gimmick.